This facility supports use of mouse models to study human diseases such as cancer, assisting with design, generation, and analysis of mice carrying engineered mutations. Services include traditional or CRISPR/Cas9 gene targeting in ES cells or embryos; coordination of pre-implantation mouse embryo injections; specialized cell and organoid culture including derivation of germline-competent ES lines and ES cell karyotyping; and assistance, training, and reagents for ES cell, organoid, embryo and mouse analysis.