Uttam RajBhandary

Uttam RajBhandary

Lester Wolfe Emeritus Professor of Molecular Biology

Before closing his lab, Uttam RajBhandary studied interactions between RNAs and proteins, focusing on gene expression and gene regulation.






  • PhD 1962, University of Durham
  • BS 1952, Chemistry, Patna University

Research Summary

Before closing his lab, Uttam RahBhandary studied RNA-protein interactions, which play a crucial role in gene expression, gene regulation and development. The many proteins with which tRNAs interact during protein synthesis make them excellent systems for investigating specific RNA-protein interactions. He examined tRNA structure, function and biosynthesis using biochemistry, genetics and in vivo functional analyses. Uttam RajBhandary is no longer accepting students.


  • American Academy of Arts and Sciences, Fellow, 1991

Key Publications

  1. Nonsense suppression in archaea. Bhattacharya, A, Köhrer, C, Mandal, D, RajBhandary, UL. 2015. Proc Natl Acad Sci U S A 112, 6015-20.
    doi: 10.1073/pnas.1501558112PMID:25918386
  2. Agmatidine, a modified cytidine in the anticodon of archaeal tRNA(Ile), base pairs with adenosine but not with guanosine. Mandal, D, Köhrer, C, Su, D, Russell, SP, Krivos, K, Castleberry, CM, Blum, P, Limbach, PA, Söll, D, RajBhandary, UL et al.. 2010. Proc Natl Acad Sci U S A 107, 2872-7.
    doi: 10.1073/pnas.0914869107PMID:20133752
  3. Suppressor mutations in Escherichia coli methionyl-tRNA formyltransferase: role of a 16-amino acid insertion module in initiator tRNA recognition. Ramesh, V, Gite, S, Li, Y, RajBhandary, UL. 1997. Proc Natl Acad Sci U S A 94, 13524-9.
    doi: 10.1073/pnas.94.25.13524PMID:9391059
  4. Initiator transfer RNAs. RajBhandary, UL. 1994. J Bacteriol 176, 547-52.
    doi: 10.1128/jb.176.3.547-552.1994PMID:7507918
  5. Initiation of protein synthesis from a termination codon. Varshney, U, RajBhandary, UL. 1990. Proc Natl Acad Sci U S A 87, 1586-90.
    doi: 10.1073/pnas.87.4.1586PMID:2406724
  6. An inducible mammalian amber suppressor: propagation of a poliovirus mutant. Sedivy, JM, Capone, JP, RajBhandary, UL, Sharp, PA. 1987. Cell 50, 379-89.
    doi: 10.1016/0092-8674(87)90492-2PMID:3038332
  7. Intron within the large rRNA gene of N. crassa mitochondria: a long open reading frame and a consensus sequence possibly important in splicing. Burke, JM, RajBhandary, UL. 1982. Cell 31, 509-20.
    doi: 10.1016/0092-8674(82)90307-5PMID:6218884
  8. Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C. Kudo, I, Leineweber, M, RajBhandary, UL. 1981. Proc Natl Acad Sci U S A 78, 4753-7.
    doi: 10.1073/pnas.78.8.4753PMID:6170979
  9. Novel features in the genetic code and codon reading patterns in Neurospora crassa mitochondria based on sequences of six mitochondrial tRNAs. Heckman, JE, Sarnoff, J, Alzner-DeWeerd, B, Yin, S, RajBhandary, UL. 1980. Proc Natl Acad Sci U S A 77, 3159-63.
    doi: 10.1073/pnas.77.6.3159PMID:6447871
  10. Structure and function of initiator methionine tRNA from the mitochondria of Neurospora crassa. Heckman, JE, Hecker, LI, Schwartzbach, SD, Barnett, WE, Baumstark, B, RajBhandary, UL. 1978. Cell 13, 83-95.
    doi: 10.1016/0092-8674(78)90140-xPMID:145896
  11. Nucleotide sequences at the 5'termini of rabbit alpha and beta globin mRNA. Lockhard, RE, Rajbhandary, UL. 1976. Cell 9, 747-60.
    doi: 10.1016/0092-8674(76)90138-0PMID:189943
  12. Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs. Simsek, M, Ziegenmeyer, J, Heckman, J, Rajbhandary, UL. 1973. Proc Natl Acad Sci U S A 70, 1041-5.
    doi: 10.1073/pnas.70.4.1041PMID:4577787