Seychelle M. Vos

Seychelle M. Vos

Robert A. Swanson (1969) Career Development Professor of Life Sciences

Seychelle M. Vos investigates how genome organization and gene expression are physically coupled across molecular scales.

617-253-5595

Phone

68-583A

Office

Meg Rheault

Assistant

617-253-1809

Assistant Phone

Education

  • PhD, 2013, University of California, Berkeley
  • BS,  2008,  Genetics,  University of Georgia

Research Summary

We study the interplay of gene expression and genome organization. Our work focuses on understanding how large molecular machineries involved in genome organization and gene transcription regulate each others’ function to ultimately determine cell fate and identity. We employ a broad range of approaches including single-particle cryo-electron microscopy (cryo-EM), X-ray crystallography, biochemistry, and genetics to mechanistically understand how these molecular assemblies regulate each other across molecular scales.

Key Publications

  1. Structure of transcribing RNA polymerase II-nucleosome complex. Farnung, L, Vos, SM, Cramer, P. 2018. Nat Commun 9, 5432.
    doi: 10.1038/s41467-018-07870-yPMID:30575770
  2. Structure of paused transcription complex Pol II-DSIF-NELF. Vos, SM, Farnung, L, Urlaub, H, Cramer, P. 2018. Nature 560, 601-606.
    doi: 10.1038/s41586-018-0442-2PMID:30135580
  3. Structure of activated transcription complex Pol II-DSIF-PAF-SPT6. Vos, SM, Farnung, L, Boehning, M, Wigge, C, Linden, A, Urlaub, H, Cramer, P. 2018. Nature 560, 607-612.
    doi: 10.1038/s41586-018-0440-4PMID:30135578
  4. Direct control of type IIA topoisomerase activity by a chromosomally encoded regulatory protein. Vos, SM, Lyubimov, AY, Hershey, DM, Schoeffler, AJ, Sengupta, S, Nagaraja, V, Berger, JM. 2014. Genes Dev 28, 1485-97.
    doi: 10.1101/gad.241984.114PMID:24990966

Recent Publications

  1. Allosteric transcription stimulation by RNA polymerase II super elongation complex. Chen, Y, Vos, SM, Dienemann, C, Ninov, M, Urlaub, H, Cramer, P. 2021. Mol Cell , .
    doi: 10.1016/j.molcel.2021.06.019PMID:34265249
  2. Understanding transcription across scales: From base pairs to chromosomes. Vos, SM. 2021. Mol Cell 81, 1601-1616.
    doi: 10.1016/j.molcel.2021.03.002PMID:33770487
  3. Stress-induced nuclear condensation of NELF drives transcriptional downregulation. Rawat, P, Boehning, M, Hummel, B, Aprile-Garcia, F, Pandit, AS, Eisenhardt, N, Khavaran, A, Niskanen, E, Vos, SM, Palvimo, JJ et al.. 2021. Mol Cell 81, 1013-1026.e11.
    doi: 10.1016/j.molcel.2021.01.016PMID:33548202
  4. Drugging the "Undruggable" MYCN Oncogenic Transcription Factor: Overcoming Previous Obstacles to Impact Childhood Cancers. Wolpaw, AJ, Bayliss, R, Büchel, G, Dang, CV, Eilers, M, Gustafson, WC, Hansen, GH, Jura, N, Knapp, S, Lemmon, MA et al.. 2021. Cancer Res 81, 1627-1632.
    doi: 10.1158/0008-5472.CAN-20-3108PMID:33509943
  5. Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promoters. Endres, T, Solvie, D, Heidelberger, JB, Andrioletti, V, Baluapuri, A, Ade, CP, Muhar, M, Eilers, U, Vos, SM, Cramer, P et al.. 2021. Mol Cell 81, 830-844.e13.
    doi: 10.1016/j.molcel.2020.12.035PMID:33453168
  6. Structure of a transcribing RNA polymerase II-U1 snRNP complex. Zhang, S, Aibara, S, Vos, SM, Agafonov, DE, Lührmann, R, Cramer, P. 2021. Science 371, 305-309.
    doi: 10.1126/science.abf1870PMID:33446560
  7. Structure of complete Pol II-DSIF-PAF-SPT6 transcription complex reveals RTF1 allosteric activation. Vos, SM, Farnung, L, Linden, A, Urlaub, H, Cramer, P. 2020. Nat Struct Mol Biol 27, 668-677.
    doi: 10.1038/s41594-020-0437-1PMID:32541898
  8. MYC Recruits SPT5 to RNA Polymerase II to Promote Processive Transcription Elongation. Baluapuri, A, Hofstetter, J, Dudvarski Stankovic, N, Endres, T, Bhandare, P, Vos, SM, Adhikari, B, Schwarz, JD, Narain, A, Vogt, M et al.. 2019. Mol Cell 74, 674-687.e11.
    doi: 10.1016/j.molcel.2019.02.031PMID:30928206
  9. Structure of transcribing RNA polymerase II-nucleosome complex. Farnung, L, Vos, SM, Cramer, P. 2018. Nat Commun 9, 5432.
    doi: 10.1038/s41467-018-07870-yPMID:30575770
  10. Structure of paused transcription complex Pol II-DSIF-NELF. Vos, SM, Farnung, L, Urlaub, H, Cramer, P. 2018. Nature 560, 601-606.
    doi: 10.1038/s41586-018-0442-2PMID:30135580

Multimedia

Photo credit: Raleigh McElvery