Seychelle M. Vos

Seychelle M. Vos

Assistant Professor of Biology

Seychelle M. Vos investigates how genome organization and gene expression are physically coupled across molecular scales.

617-253-5595

Phone

68-583A

Office

Meg Rheault

Assistant

617-253-1809

Assistant Phone

Education

  • PhD, 2013, University of California, Berkeley
  • BS,  2008,  Genetics,  University of Georgia

Research Summary

We study the interplay of gene expression and genome organization. Our work focuses on understanding how large molecular machineries involved in genome organization and gene transcription regulate each others’ function to ultimately determine cell fate and identity. We employ a broad range of approaches including single-particle cryo-electron microscopy (cryo-EM), X-ray crystallography, biochemistry, and genetics to mechanistically understand how these molecular assemblies regulate each other across molecular scales.

Key Publications

  1. Structure of transcribing RNA polymerase II-nucleosome complex. Farnung, L, Vos, SM, Cramer, P. 2018. Nat Commun 9, 5432.
    doi: 10.1038/s41467-018-07870-yPMID:30575770
  2. Structure of paused transcription complex Pol II-DSIF-NELF. Vos, SM, Farnung, L, Urlaub, H, Cramer, P. 2018. Nature 560, 601-606.
    doi: 10.1038/s41586-018-0442-2PMID:30135580
  3. Structure of activated transcription complex Pol II-DSIF-PAF-SPT6. Vos, SM, Farnung, L, Boehning, M, Wigge, C, Linden, A, Urlaub, H, Cramer, P. 2018. Nature 560, 607-612.
    doi: 10.1038/s41586-018-0440-4PMID:30135578
  4. Direct control of type IIA topoisomerase activity by a chromosomally encoded regulatory protein. Vos, SM, Lyubimov, AY, Hershey, DM, Schoeffler, AJ, Sengupta, S, Nagaraja, V, Berger, JM. 2014. Genes Dev. 28, 1485-97.
    doi: 10.1101/gad.241984.114PMID:24990966

Recent Publications

  1. MYC Recruits SPT5 to RNA Polymerase II to Promote Processive Transcription Elongation. Baluapuri, A, Hofstetter, J, Dudvarski Stankovic, N, Endres, T, Bhandare, P, Vos, SM, Adhikari, B, Schwarz, JD, Narain, A, Vogt, M et al.. 2019. Mol. Cell 74, 674-687.e11.
    doi: 10.1016/j.molcel.2019.02.031PMID:30928206
  2. Structure of transcribing RNA polymerase II-nucleosome complex. Farnung, L, Vos, SM, Cramer, P. 2018. Nat Commun 9, 5432.
    doi: 10.1038/s41467-018-07870-yPMID:30575770
  3. Structure of paused transcription complex Pol II-DSIF-NELF. Vos, SM, Farnung, L, Urlaub, H, Cramer, P. 2018. Nature 560, 601-606.
    doi: 10.1038/s41586-018-0442-2PMID:30135580
  4. Structure of activated transcription complex Pol II-DSIF-PAF-SPT6. Vos, SM, Farnung, L, Boehning, M, Wigge, C, Linden, A, Urlaub, H, Cramer, P. 2018. Nature 560, 607-612.
    doi: 10.1038/s41586-018-0440-4PMID:30135578
  5. Nucleosome-Chd1 structure and implications for chromatin remodelling. Farnung, L, Vos, SM, Wigge, C, Cramer, P. 2017. Nature 550, 539-542.
    doi: 10.1038/nature24046PMID:29019976
  6. RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases. Battaglia, S, Lidschreiber, M, Baejen, C, Torkler, P, Vos, SM, Cramer, P. 2017. Elife 6, .
    doi: 10.7554/eLife.25637PMID:28537551
  7. Architecture and RNA binding of the human negative elongation factor. Vos, SM, Pöllmann, D, Caizzi, L, Hofmann, KB, Rombaut, P, Zimniak, T, Herzog, F, Cramer, P. 2016. Elife 5, .
    doi: 10.7554/eLife.14981PMID:27282391
  8. Direct control of type IIA topoisomerase activity by a chromosomally encoded regulatory protein. Vos, SM, Lyubimov, AY, Hershey, DM, Schoeffler, AJ, Sengupta, S, Nagaraja, V, Berger, JM. 2014. Genes Dev. 28, 1485-97.
    doi: 10.1101/gad.241984.114PMID:24990966
  9. Structural basis for the MukB-topoisomerase IV interaction and its functional implications in vivo. Vos, SM, Stewart, NK, Oakley, MG, Berger, JM. 2013. EMBO J. 32, 2950-62.
    doi: 10.1038/emboj.2013.218PMID:24097060
  10. Distinct regions of the Escherichia coli ParC C-terminal domain are required for substrate discrimination by topoisomerase IV. Vos, SM, Lee, I, Berger, JM. 2013. J. Mol. Biol. 425, 3029-45.
    doi: 10.1016/j.jmb.2013.04.033PMID:23867279
Photo credit: Raleigh McElvery