Sebastian Lourido

Sebastian Lourido

Latham Family Career Development Professor; Core Member, Whitehead Institute

Sebastian Lourido exposes parasite vulnerabilities and harnesses them to treat infectious disease.





Gabrielle McCauley



Assistant Phone


  • PhD, 2012, Washington University in St. Louis
  • BS, 2004, Cellular and Molecular Biology and Studio Art, Tulane University

Research Summary

Our lab is interested in the molecular events that enable apicomplexan parasites to remain widespread and deadly infectious agents. We study many important human pathogens, including Toxoplasma gondii, to model features conserved throughout the phylum. We seek to expand our understanding of eukaryotic diversity and identify specific features that can be targeted to treat parasite infections.


  • Odyssey Award, Smith Family Foundation, 2021

Recent Publications

  1. Screening the Toxoplasma kinome with high-throughput tagging identifies a regulator of invasion and egress. Smith, TA, Lopez-Perez, GS, Herneisen, AL, Shortt, E, Lourido, S. 2022. Nat Microbiol , .
    doi: 10.1038/s41564-022-01104-0PMID:35484233
  2. Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins. Rivera-Cuevas, Y, Mayoral, J, Di Cristina, M, Lawrence, AE, Olafsson, EB, Patel, RK, Thornhill, D, Waldman, BS, Ono, A, Sexton, JZ et al.. 2021. PLoS Pathog 17, e1010138.
    doi: 10.1371/journal.ppat.1010138PMID:34898650
  3. Thermal Proteome Profiling to Identify Protein-ligand Interactions in the Apicomplexan Parasite Toxoplasma gondii. Herneisen, AL, Lourido, S. 2021. Bio Protoc 11, e4207.
    doi: 10.21769/BioProtoc.4207PMID:34859122
  4. CRISPR-Mediated Transcriptional Repression in Toxoplasma gondii. Markus, BM, Boydston, EA, Lourido, S. 2021. mSphere 6, e0047421.
    doi: 10.1128/mSphere.00474-21PMID:34643425
  5. High-Resolution Mapping of Transcription Initiation in the Asexual Stages of Toxoplasma gondii. Markus, BM, Waldman, BS, Lorenzi, HA, Lourido, S. 2020. Front Cell Infect Microbiol 10, 617998.
    doi: 10.3389/fcimb.2020.617998PMID:33553008
  6. Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFNγ-activated murine macrophages. Wang, Y, Sangaré, LO, Paredes-Santos, TC, Hassan, MA, Krishnamurthy, S, Furuta, AM, Markus, BM, Lourido, S, Saeij, JPJ. 2020. Nat Commun 11, 5258.
    doi: 10.1038/s41467-020-18991-8PMID:33067458
  7. Genetic screens reveal a central role for heme metabolism in artemisinin susceptibility. Harding, CR, Sidik, SM, Petrova, B, Gnädig, NF, Okombo, J, Herneisen, AL, Ward, KE, Markus, BM, Boydston, EA, Fidock, DA et al.. 2020. Nat Commun 11, 4813.
    doi: 10.1038/s41467-020-18624-0PMID:32968076
  8. Identifying the Target of an Antiparasitic Compound in Toxoplasma Using Thermal Proteome Profiling. Herneisen, AL, Sidik, SM, Markus, BM, Drewry, DH, Zuercher, WJ, Lourido, S. 2020. ACS Chem Biol 15, 1801-1807.
    doi: 10.1021/acschembio.0c00369PMID:32597628
  9. A PEROXO-Tag Enables Rapid Isolation of Peroxisomes from Human Cells. Ray, GJ, Boydston, EA, Shortt, E, Wyant, GA, Lourido, S, Chen, WW, Sabatini, DM. 2020. iScience 23, 101109.
    doi: 10.1016/j.isci.2020.101109PMID:32417403
  10. Functional and Computational Genomics Reveal Unprecedented Flexibility in Stage-Specific Toxoplasma Metabolism. Krishnan, A, Kloehn, J, Lunghi, M, Chiappino-Pepe, A, Waldman, BS, Nicolas, D, Varesio, E, Hehl, A, Lourido, S, Hatzimanikatis, V et al.. 2020. Cell Host Microbe 27, 290-306.e11.
    doi: 10.1016/j.chom.2020.01.002PMID:31991093
More Publications






Photo credit: Gretchen Ertl/Whitehead Institute