Sebastian Lourido

Sebastian Lourido

Assistant Professor of Biology; Member, Whitehead Institute

Sebastian Lourido exposes parasite vulnerabilities and harnesses them to treat infectious disease.

617-324-4920

Phone

WI-561D

Office

Education

PhD 2012, Washington University in St. Louis

Research Summary

Our lab is interested in the molecular events that enable apicomplexan parasites to remain widespread and deadly infectious agents. We study many important human pathogens, including Toxoplasma gondii, to model features conserved throughout the phylum. We seek to expand our understanding of eukaryotic diversity and identify specific features that can be targeted to treat parasite infections.

Recent Publications

  1. Toxoplasma CRISPR/Cas9 constructs are functional for gene disruption in Neospora caninum. Arranz-Solís, D, Regidor-Cerrillo, J, Lourido, S, Ortega-Mora, LM, Saeij, JPJ. 2018. Int. J. Parasitol. 48, 597-600.
    doi: 10.1016/j.ijpara.2018.03.002PMID:29625127
  2. Identification of PNG kinase substrates uncovers interactions with the translational repressor TRAL in the oocyte-to-embryo transition. Hara, M, Lourido, S, Petrova, B, Lou, HJ, Von Stetina, JR, Kashevsky, H, Turk, BE, Orr-Weaver, TL. 2018. Elife 7, .
    doi: 10.7554/eLife.33150PMID:29480805
  3. CRISPR-Cas9-based genome-wide screening of Toxoplasma gondii. Sidik, SM, Huet, D, Lourido, S. 2018. Nat Protoc 13, 307-323.
    doi: 10.1038/nprot.2017.131PMID:29323662
  4. O-fucosylated glycoproteins form assemblies in close proximity to the nuclear pore complexes of Toxoplasma gondii. Bandini, G, Haserick, JR, Motari, E, Ouologuem, DT, Lourido, S, Roos, DS, Costello, CE, Robbins, PW, Samuelson, J. 2016. Proc. Natl. Acad. Sci. U.S.A. 113, 11567-11572.
    doi: 10.1073/pnas.1613653113PMID:27663739
  5. A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, SM, Huet, D, Ganesan, SM, Huynh, MH, Wang, T, Nasamu, AS, Thiru, P, Saeij, JPJ, Carruthers, VB, Niles, JC et al.. 2016. Cell 166, 1423-1435.e12.
    doi: 10.1016/j.cell.2016.08.019PMID:27594426
  6. Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose. Chahal, JS, Khan, OF, Cooper, CL, McPartlan, JS, Tsosie, JK, Tilley, LD, Sidik, SM, Lourido, S, Langer, R, Bavari, S et al.. 2016. Proc. Natl. Acad. Sci. U.S.A. 113, E4133-42.
    doi: 10.1073/pnas.1600299113PMID:27382155
  7. Serum Albumin Stimulates Protein Kinase G-dependent Microneme Secretion in Toxoplasma gondii. Brown, KM, Lourido, S, Sibley, LD. 2016. J. Biol. Chem. 291, 9554-65.
    doi: 10.1074/jbc.M115.700518PMID:26933037
  8. Using a Genetically Encoded Sensor to Identify Inhibitors of Toxoplasma gondii Ca2+ Signaling. Sidik, SM, Hortua Triana, MA, Paul, AS, El Bakkouri, M, Hackett, CG, Tran, F, Westwood, NJ, Hui, R, Zuercher, WJ, Duraisingh, MT et al.. 2016. J. Biol. Chem. 291, 9566-80.
    doi: 10.1074/jbc.M115.703546PMID:26933036
  9. Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles. Rothenberg, DA, Gordon, EA, White, FM, Lourido, S. 2016. Methods Mol. Biol. 1355, 71-84.
    doi: 10.1007/978-1-4939-3049-4_5PMID:26584919
  10. Allosteric activation of apicomplexan calcium-dependent protein kinases. Ingram, JR, Knockenhauer, KE, Markus, BM, Mandelbaum, J, Ramek, A, Shan, Y, Shaw, DE, Schwartz, TU, Ploegh, HL, Lourido, S et al.. 2015. Proc. Natl. Acad. Sci. U.S.A. 112, E4975-84.
    doi: 10.1073/pnas.1505914112PMID:26305940
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Photo credit: Gretchen Ertl/Whitehead Institute