Kristin Knouse

Kristin Knouse

Whitehead Career Development Professor; Intramural Faculty, Koch Institute

Kristin Knouse seeks to understand and modulate organ injury and repair by innovating tools for experimentation directly within living organisms.

76-561D

Office

knouse@mit.edu

Email

Koch Institute for Integrative Cancer Research

Location

Lab Website
Tony McMillen

Assistant

520-784-1810

Assistant Phone

Education

  • PhD, 2017, MIT; MD, 2018, Harvard Medical School
  • Undergraduate: BS, 2010, Biology, Duke University

Research Summary

We aim to understand how tissues sense and respond to damage with the goal of developing novel treatments for diverse human diseases. We focus on the mammalian liver, which has the unique ability to completely regenerate itself, in order to identify the molecular requirements for effective organ repair. To this end, we innovate genetic, molecular, and cellular tools that allow us to investigate and modulate organ injury and regeneration directly within living organisms.

Awards

  • NIH Director’s Early Independence Award, 2018
  • Henry Asbury Christian Award, 2018

Key Publications

  1. Genome-scale CRISPR screening in a single mouse liver. Keys, HR, Knouse, KA. 2022. Cell Genom 2, .
    doi: 10.1016/j.xgen.2022.100217PMID:36643909
  2. Quiescent Cells Actively Replenish CENP-A Nucleosomes to Maintain Centromere Identity and Proliferative Potential. Swartz, SZ, McKay, LS, Su, KC, Bury, L, Padeganeh, A, Maddox, PS, Knouse, KA, Cheeseman, IM. 2019. Dev Cell 51, 35-48.e7.
    doi: 10.1016/j.devcel.2019.07.016PMID:31422918
  3. Chromosome Segregation Fidelity in Epithelia Requires Tissue Architecture. Knouse, KA, Lopez, KE, Bachofner, M, Amon, A. 2018. Cell 175, 200-211.e13.
    doi: 10.1016/j.cell.2018.07.042PMID:30146160
  4. Assessment of megabase-scale somatic copy number variation using single-cell sequencing. Knouse, KA, Wu, J, Amon, A. 2016. Genome Res 26, 376-84.
    doi: 10.1101/gr.198937.115PMID:26772196
  5. Single cell sequencing reveals low levels of aneuploidy across mammalian tissues. Knouse, KA, Wu, J, Whittaker, CA, Amon, A. 2014. Proc Natl Acad Sci U S A 111, 13409-14.
    doi: 10.1073/pnas.1415287111PMID:25197050

Recent Publications

  1. Genome-scale CRISPR screening in a single mouse liver. Keys, HR, Knouse, KA. 2022. Cell Genom 2, .
    doi: 10.1016/j.xgen.2022.100217PMID:36643909
  2. Quiescent Cells Actively Replenish CENP-A Nucleosomes to Maintain Centromere Identity and Proliferative Potential. Swartz, SZ, McKay, LS, Su, KC, Bury, L, Padeganeh, A, Maddox, PS, Knouse, KA, Cheeseman, IM. 2019. Dev Cell 51, 35-48.e7.
    doi: 10.1016/j.devcel.2019.07.016PMID:31422918
  3. Chromosome Segregation Fidelity in Epithelia Requires Tissue Architecture. Knouse, KA, Lopez, KE, Bachofner, M, Amon, A. 2018. Cell 175, 200-211.e13.
    doi: 10.1016/j.cell.2018.07.042PMID:30146160
  4. In situ expansion of engineered human liver tissue in a mouse model of chronic liver disease. Stevens, KR, Scull, MA, Ramanan, V, Fortin, CL, Chaturvedi, RR, Knouse, KA, Xiao, JW, Fung, C, Mirabella, T, Chen, AX et al.. 2017. Sci Transl Med 9, .
    doi: 10.1126/scitranslmed.aah5505PMID:28724577
  5. Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System. Santaguida, S, Richardson, A, Iyer, DR, M'Saad, O, Zasadil, L, Knouse, KA, Wong, YL, Rhind, N, Desai, A, Amon, A et al.. 2017. Dev Cell 41, 638-651.e5.
    doi: 10.1016/j.devcel.2017.05.022PMID:28633018
  6. Detection of Copy Number Alterations Using Single Cell Sequencing. Knouse, KA, Wu, J, Hendricks, A. 2017. J Vis Exp , .
    doi: 10.3791/55143PMID:28287554
  7. Aneuploidy impairs hematopoietic stem cell fitness and is selected against in regenerating tissues in vivo. Pfau, SJ, Silberman, RE, Knouse, KA, Amon, A. 2016. Genes Dev 30, 1395-408.
    doi: 10.1101/gad.278820.116PMID:27313317
  8. Assessment of megabase-scale somatic copy number variation using single-cell sequencing. Knouse, KA, Wu, J, Amon, A. 2016. Genome Res 26, 376-84.
    doi: 10.1101/gr.198937.115PMID:26772196
  9. Cell biology: the micronucleus gets its big break. Knouse, KA, Amon, A. 2015. Nature 522, 162-3.
    doi: 10.1038/nature14528PMID:26017308
  10. Single cell sequencing reveals low levels of aneuploidy across mammalian tissues. Knouse, KA, Wu, J, Whittaker, CA, Amon, A. 2014. Proc Natl Acad Sci U S A 111, 13409-14.
    doi: 10.1073/pnas.1415287111PMID:25197050
  11. A genome-wide screen in the mouse liver reveals sex-specific and cell non-autonomous regulation of cell fitness. Keys, H.R. and Knouse, K.A. 2021. bioRxiv.
    doi: https://doi.org/10.1101/2021.01.30.428976
More Publications

Multimedia

 

Photo credit: Gretchen Ertl/Whitehead Institute