JoAnne Stubbe

JoAnne Stubbe

Professor Emerita

JoAnne Stubbe studies ribonucleotide reductases — essential enzymes that provide the building blocks for DNA replication, repair and successful targets of multiple clinical drugs.






Assistant Phone


  • PhD, 1971, University of California, Berkeley
  • BA, 1968, Chemistry, University of Pennsylvania

Research Summary

I study ribonucleotide reductases (RNRs), which catalyze the conversion of nucleotides to deoxynucleotides and play an essential role in DNA replication and repair. I support a range of ongoing projects, including those related to the radical propagation pathway utilized by Class I RNRs, the interactions between protein subunits of Class I RNR, the regulation of RNRs, and the mechanisms behind clinical drugs. I also examine the biosynthesis, activation, and regulation of formation of the essential metallo-cofactors of RNRs in E. coli, S. cerevisiae, and humans. JoAnne Stubbe is no longer accepting students.


  • Priestley Medalist, American Chemical Society, 2020
  • American Association for the Advancement of Science, Fellow, 2014
  • National Science Foundation, National Medal of Science, 2008
  • National Academy of Sciences, Member, 1992
  • American Academy of Arts and Sciences, Fellow, 1991
  • Welch Award in Chemistry 2010
  • NAS Chemical Sciences Award 2010
  • Pearl Meister Greengard Award 2017

Key Publications

  1. Conformationally Dynamic Radical Transfer within Ribonucleotide Reductase. Greene, BL, Taguchi, AT, Stubbe, J, Nocera, DG. 2017. J. Am. Chem. Soc. 139, 16657-16665.
    doi: 10.1021/jacs.7b08192PMID:29037038
  2. Spectroscopic Evidence for a H Bond Network at Y356 Located at the Subunit Interface of Active E. coli Ribonucleotide Reductase. Nick, TU, Ravichandran, KR, Stubbe, J, Kasanmascheff, M, Bennati, M. 2017. Biochemistry 56, 3647-3656.
    doi: 10.1021/acs.biochem.7b00462PMID:28640584
  3. Glutamate 52-β at the α/β subunit interface of Escherichia coli class Ia ribonucleotide reductase is essential for conformational gating of radical transfer. Lin, Q, Parker, MJ, Taguchi, AT, Ravichandran, K, Kim, A, Kang, G, Shao, J, Drennan, CL, Stubbe, J. 2017. J. Biol. Chem. 292, 9229-9239.
    doi: 10.1074/jbc.M117.783092PMID:28377505
  4. Formal Reduction Potentials of Difluorotyrosine and Trifluorotyrosine Protein Residues: Defining the Thermodynamics of Multistep Radical Transfer. Ravichandran, KR, Zong, AB, Taguchi, AT, Nocera, DG, Stubbe, J, Tommos, C. 2017. J. Am. Chem. Soc. 139, 2994-3004.
    doi: 10.1021/jacs.6b11011PMID:28171730
  5. Glutamate 350 Plays an Essential Role in Conformational Gating of Long-Range Radical Transport in Escherichia coli Class Ia Ribonucleotide Reductase. Ravichandran, K, Minnihan, EC, Lin, Q, Yokoyama, K, Taguchi, AT, Shao, J, Nocera, DG, Stubbe, J. 2017. Biochemistry 56, 856-868.
    doi: 10.1021/acs.biochem.6b01145PMID:28103007

Recent Publications

  1. Conformational Motions and Water Networks at the α/β Interface in E. coli Ribonucleotide Reductase. Reinhardt, CR, Li, P, Kang, G, Stubbe, J, Drennan, CL, Hammes-Schiffer, S. 2020. J. Am. Chem. Soc. 142, 13768-13778.
    doi: 10.1021/jacs.0c04325PMID:32631052
  2. Ribonucleotide Reductases: Structure, Chemistry, and Metabolism Suggest New Therapeutic Targets. Greene, BL, Kang, G, Cui, C, Bennati, M, Nocera, DG, Drennan, CL, Stubbe, J. 2020. Annu. Rev. Biochem. 89, 45-75.
    doi: 10.1146/annurev-biochem-013118-111843PMID:32569524
  3. PET Polymer Recycling. O'Reilly, M, Stubbe, J. 2020. Biochemistry 59, 2316-2318.
    doi: 10.1021/acs.biochem.0c00457PMID:32559062
  4. Structure of a trapped radical transfer pathway within a ribonucleotide reductase holocomplex. Kang, G, Taguchi, AT, Stubbe, J, Drennan, CL. 2020. Science 368, 424-427.
    doi: 10.1126/science.aba6794PMID:32217749
  5. Subunit Interaction Dynamics of Class Ia Ribonucleotide Reductases: In Search of a Robust Assay. Ravichandran, K, Olshansky, L, Nocera, DG, Stubbe, J. 2020. Biochemistry 59, 1442-1453.
    doi: 10.1021/acs.biochem.0c00001PMID:32186371
  6. Convergent allostery in ribonucleotide reductase. Thomas, WC, Brooks, FP 3rd, Burnim, AA, Bacik, JP, Stubbe, J, Kaelber, JT, Chen, JZ, Ando, N. 2019. Nat Commun 10, 2653.
    doi: 10.1038/s41467-019-10568-4PMID:31201319
  7. Discovery of a New Class I Ribonucleotide Reductase with an Essential DOPA Radical and NO Metal as an Initiator of Long-Range Radical Transfer. Stubbe, J, Seyedsayamdost, MR. 2019. Biochemistry 58, 435-437.
    doi: 10.1021/acs.biochem.8b01238PMID:30586288
  8. Basis of dATP inhibition of RNRs. Greene, BL, Nocera, DG, Stubbe, J. 2018. J. Biol. Chem. 293, 10413-10414.
    doi: 10.1074/jbc.H118.003717PMID:29959279
  9. An endogenous dAMP ligand in Bacillus subtilis class Ib RNR promotes assembly of a noncanonical dimer for regulation by dATP. Parker, MJ, Maggiolo, AO, Thomas, WC, Kim, A, Meisburger, SP, Ando, N, Boal, AK, Stubbe, J. 2018. Proc. Natl. Acad. Sci. U.S.A. 115, E4594-E4603.
    doi: 10.1073/pnas.1800356115PMID:29712847
  10. 3.3-Å resolution cryo-EM structure of human ribonucleotide reductase with substrate and allosteric regulators bound. Brignole, EJ, Tsai, KL, Chittuluru, J, Li, H, Aye, Y, Penczek, PA, Stubbe, J, Drennan, CL, Asturias, F. 2018. Elife 7, .
    doi: 10.7554/eLife.31502PMID:29460780
More Publications



Photo Credit: Justin Knight