Daniel Cham-Chin Lim

Daniel Cham-Chin Lim

Manager, Crystallography Lab

Daniel Cham-Chin Lim manage the instrumentation within the Structural Biology Core Facility, and provides training and support to the users of the facility.

617-452-3472

Phone

68-580b

Office

danlim@mit.edu

Email

Summary

Daniel earned his PhD from the University of British Columbia, where he investigated the structure of bacterial proteins involved in antibiotic resistance. He went on to complete his postdoctoral studies at MIT's Koch Institute For Integrative Cancer Research in the lab of Michael Yaffe. Now, as the manager of the Crystallography Lab in Building 68, he performs routine maintenance on the facility’s instrumentation (robotics, X-ray generator, and ancillary equipment), and deals with outside vendors when non-routine repairs are required. He also trains facility users to operate the instrumentation, and instructs them in all aspects of macromolecular crystallography — including screening for crystallization conditions, optimizing crystal growth, preparing crystals for data collection, collecting X-ray data on the MIT home source or at the APS synchrotron, processing X-ray data, and structure solution.

Fellowships

  • Schering Plough Research Institute / MIT Biology Postdoctoral Fellow, 2010-2012
  • Canadian Institutes for Health Research Postdoctoral Fellow, 2006-2008
  • Jane Coffin Childs Postdoctoral Fellow, 2003-2006

Recent Publications

  1. Are redox changes a critical switch for mitotic progression? Lim, DC, Joukov, V, Yaffe, MB. 2020. Mol Cell Oncol 7, 1832419.
    doi: 10.1080/23723556.2020.1832419PMID:33235921
  2. Redox priming promotes Aurora A activation during mitosis. Lim, DC, Joukov, V, Rettenmaier, TJ, Kumagai, A, Dunphy, WG, Wells, JA, Yaffe, MB. 2020. Sci Signal 13, .
    doi: 10.1126/scisignal.abb6707PMID:32694171
  3. Structure of the Toxoplasma gondii ROP18 kinase domain reveals a second ligand binding pocket required for acute virulence. Lim, D, Gold, DA, Julien, L, Rosowski, EE, Niedelman, W, Yaffe, MB, Saeij, JP. 2013. J Biol Chem 288, 34968-80.
    doi: 10.1074/jbc.M113.523266PMID:24129568
  4. Structural basis for the beta lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus. Lim, D, Strynadka, NC. 2002. Nat Struct Biol 9, 870-6.
    doi: 10.1038/nsb858PMID:12389036
  5. Heavy metal-mediated crystallization of Escherichia coli phytase and analysis of bridging interactions. Lim, D, Jia, Z. 2002. Protein Pept Lett 9, 359-65.
    doi: 10.2174/0929866023408634PMID:12144514
  6. Crystal structure of the MexR repressor of the mexRAB-oprM multidrug efflux operon of Pseudomonas aeruginosa. Lim, D, Poole, K, Strynadka, NC. 2002. J Biol Chem 277, 29253-9.
    doi: 10.1074/jbc.M111381200PMID:12034710
  7. Crystal structure and kinetic analysis of beta-lactamase inhibitor protein-II in complex with TEM-1 beta-lactamase. Lim, D, Park, HU, De Castro, L, Kang, SG, Lee, HS, Jensen, S, Lee, KJ, Strynadka, NC. 2001. Nat Struct Biol 8, 848-52.
    doi: 10.1038/nsb1001-848PMID:11573088
  8. Insights into the molecular basis for the carbenicillinase activity of PSE-4 beta-lactamase from crystallographic and kinetic studies. Lim, D, Sanschagrin, F, Passmore, L, De Castro, L, Levesque, RC, Strynadka, NC. 2001. Biochemistry 40, 395-402.
    doi: 10.1021/bi001653vPMID:11148033
  9. Crystal structures of Escherichia coli phytase and its complex with phytate. Lim, D, Golovan, S, Forsberg, CW, Jia, Z. 2000. Nat Struct Biol 7, 108-13.
    doi: 10.1038/72371PMID:10655611