EducationPhD 1998, University of California, Los Angeles
Research SummaryOur goal is to understand, at a high level of detail, how the interaction properties of proteins are encoded in their sequences and structures. We investigate protein-protein interactions by integrating data from high throughput assays, structural modeling, and bioinformatics with biochemical and biophysical experiments. Much of our work focuses on α-helical coiled-coil proteins, Bcl-2 apoptosis-regulating proteins, and protein domains that bind to short linear motifs.
- Epistatic mutations in PUMA BH3 drive an alternate binding mode to potently and selectively inhibit anti-apoptotic Bfl-1. Jenson, JM, Ryan, JA, Grant, RA, Letai, A, Keating, AE. 2017. Elife 6, .
- Data-driven prediction and design of bZIP coiled-coil interactions. Potapov, V, Kaplan, JB, Keating, AE. 2015. PLoS Comput. Biol. 11, e1004046.
- SORTCERY-A High-Throughput Method to Affinity Rank Peptide Ligands. Reich, LL, Dutta, S, Keating, AE. 2015. J. Mol. Biol. 427, 2135-50.
- Designed BH3 peptides with high affinity and specificity for targeting Mcl-1 in cells. Foight, GW, Ryan, JA, Gullá, SV, Letai, A, Keating, AE. 2014. ACS Chem. Biol. 9, 1962-8.
- Networks of bZIP protein-protein interactions diversified over a billion years of evolution. Reinke, AW, Baek, J, Ashenberg, O, Keating, AE. 2013. Science 340, 730-4.