Amy E. Keating

Amy E. Keating

Jay A. Stein (1968) Professor of Biology; Professor of Biological Engineering; Department Head

Amy E. Keating determines how proteins make specific interactions with one another and designs new, synthetic protein-protein interactions.

617-452-3398

Phone

68-622A

Office

keating@mit.edu

Email

Building 68 - Koch Biology Building

Location

Lab Website
Keith Murray

Assistant

617-452-2717

Assistant Phone

Education

  • PhD, 1998, University of California, Los Angeles
  • SB, 1992, Physics, Harvard University

Research Summary

Our goal is to understand, at a high level of detail, how the interaction properties of proteins are encoded in their sequences and structures. We investigate protein-protein interactions by integrating data from high throughput assays, structural modeling, and bioinformatics with biochemical and biophysical experiments. Much of our work focuses on α-helical coiled-coil proteins, Bcl-2 apoptosis-regulating proteins, and protein domains that bind to short linear motifs.

Recent Publications

  1. STAT5B leukemic mutations, altering SH2 tyrosine 665, have opposing impacts on immune gene programs. Lee, HK, Chen, J, Philips, RL, Lee, SG, Feng, X, Wu, Z, Liu, C, Schultz, AB, Dalzell, M, Meggendorfer, M et al.. 2025. Life Sci Alliance 8, .
    doi: 10.26508/lsa.202503222PMID:40228864
  2. High-throughput discovery of inhibitory protein fragments with AlphaFold. Savinov, A, Swanson, S, Keating, AE, Li, GW. 2025. Proc Natl Acad Sci U S A 122, e2322412122.
    doi: 10.1073/pnas.2322412122PMID:39899719
  3. STAT5B leukemic mutations, altering SH2 tyrosine 665, have opposing impacts on immune gene programs. Lee, HK, Chen, J, Philips, RL, Lee, SG, Feng, X, Wu, Z, Liu, C, Schultz, AB, Dalzell, M, Birnbaum, F et al.. 2024. bioRxiv , .
    doi: 10.1101/2024.12.20.629685PMID:39803507
  4. Elaboration of the Homer1 recognition landscape reveals incomplete divergence of paralogous EVH1 domains. Singer, A, Ramos, A, Keating, AE. 2024. Protein Sci 33, e5094.
    doi: 10.1002/pro.5094PMID:38989636
  5. High-throughput discovery of inhibitory protein fragments with AlphaFold. Savinov, A, Swanson, S, Keating, AE, Li, GW. 2024. bioRxiv , .
    doi: 10.1101/2023.12.19.572389PMID:38187731
  6. Marginal specificity in protein interactions constrains evolution of a paralogous family. Ghose, DA, Przydzial, KE, Mahoney, EM, Keating, AE, Laub, MT. 2023. Proc Natl Acad Sci U S A 120, e2221163120.
    doi: 10.1073/pnas.2221163120PMID:37098061
  7. Peptides from human BNIP5 and PXT1 and non-native binders of pro-apoptotic BAK can directly activate or inhibit BAK-mediated membrane permeabilization. Aguilar, F, Yu, S, Grant, RA, Swanson, S, Ghose, D, Su, BG, Sarosiek, KA, Keating, AE. 2023. Structure 31, 265-281.e7.
    doi: 10.1016/j.str.2023.01.001PMID:36706751
  8. Neural network-derived Potts models for structure-based protein design using backbone atomic coordinates and tertiary motifs. Li, AJ, Lu, M, Desta, I, Sundar, V, Grigoryan, G, Keating, AE. 2023. Protein Sci 32, e4554.
    doi: 10.1002/pro.4554PMID:36564857
  9. Tertiary motifs as building blocks for the design of protein-binding peptides. Swanson, S, Sivaraman, V, Grigoryan, G, Keating, AE. 2022. Protein Sci 31, e4322.
    doi: 10.1002/pro.4322PMID:35634780
  10. Native proline-rich motifs exploit sequence context to target actin-remodeling Ena/VASP protein ENAH. Hwang, T, Parker, SS, Hill, SM, Grant, RA, Ilunga, MW, Sivaraman, V, Mouneimne, G, Keating, AE. 2022. Elife 11, .
    doi: 10.7554/eLife.70680PMID:35076015
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