Alan D. Grossman

Alan D. Grossman

Praecis Professor of Biology; Department Head

Alan Grossman studies mechanisms and regulation of DNA replication, gene expression, and horizontal gene transfer in bacteria.

617-253-1515

Phone

68-530

Office

adg@mit.edu

Email

Gina Lee

Assistant

617-258-6473

Assistant Phone

Education

PhD 1984, University of Wisconsin, Madison

Research Summary

We use a variety of approaches to investigate several of the fundamental and conserved processes used by bacteria for propagation and growth, adaptation to stresses, and acquisition of new genes and traits via horizontal gene transfer. Our long term goals are to understand many of the molecular mechanisms and regulation underlying basic cellular processes in bacteria. Our organism of choice for these studies is usually the Gram positive bacterium Bacillus subtilis. Our current efforts are focused in two important areas of biology: 1) The control of horizontal gene transfer, specifically the lifecycle, function, and control of integrative and conjugative elements (ICEs). These elements are widespread in bacteria and contribute greatly to the spread of antibiotic resistances between organisms. 2) Regulation of the initiation of DNA replication and the connections between replication and gene expression, with particular focus on the conserved replication initiator and transcription factor DnaA. This work is directly related to mechanisms controlling bacterial growth, survival, and stress responses.

Awards

  • National Academy of Sciences, 2014
  • American Academy of Arts and Sciences, 2008
  • American Academy of Microbiology 1998
  • Eli Lilly Company Research Award, 1997

Key Publications

  1. Integrative and Conjugative Elements (ICEs): What They Do and How They Work. Johnson, CM, Grossman, AD. 2015. Annu. Rev. Genet. 49, 577-601.
    doi: 10.1146/annurev-genet-112414-055018PMID:26473380
  2. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA. Goranov, AI, Katz, L, Breier, AM, Burge, CB, Grossman, AD. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 12932-7.
    doi: 10.1073/pnas.0506174102PMID:16120674
  3. Regulation of a Bacillus subtilis mobile genetic element by intercellular signaling and the global DNA damage response. Auchtung, JM, Lee, CA, Monson, RE, Lehman, AP, Grossman, AD. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 12554-9.
    doi: 10.1073/pnas.0505835102PMID:16105942
  4. Localization of bacterial DNA polymerase: evidence for a factory model of replication. Lemon, KP, Grossman, AD. 1998. Science 282, 1516-9.
    PMID:9822387
  5. Biochemical and genetic characterization of a competence pheromone from B. subtilis. Magnuson, R, Solomon, J, Grossman, AD. 1994. Cell 77, 207-16.
    PMID:8168130

Recent Publications

  1. Genetic networks controlled by the bacterial replication initiator and transcription factor DnaA in Bacillus subtilis. Washington, TA, Smith, JL, Grossman, AD. 2017. Mol. Microbiol. 106, 109-128.
    doi: 10.1111/mmi.13755PMID:28752667
  2. Genetic and biochemical interactions between the bacterial replication initiator DnaA and the nucleoid-associated protein Rok in Bacillus subtilis. Seid, CA, Smith, JL, Grossman, AD. 2017. Mol. Microbiol. 103, 798-817.
    doi: 10.1111/mmi.13590PMID:27902860
  3. Autonomous Replication of the Conjugative Transposon Tn916. Wright, LD, Grossman, AD. 2016. J. Bacteriol. 198, 3355-3366.
    doi: 10.1128/JB.00639-16PMID:27698087
  4. Complete Genome Sequence of Bacillus subtilis Strain CU1050, Which Is Sensitive to Phage SPβ. Johnson, CM, Grossman, AD. 2016. Genome Announc 4, .
    doi: 10.1128/genomeA.00262-16PMID:27056236
  5. The Composition of the Cell Envelope Affects Conjugation in Bacillus subtilis. Johnson, CM, Grossman, AD. 2016. J. Bacteriol. 198, 1241-9.
    doi: 10.1128/JB.01044-15PMID:26833415
  6. Identification of host genes that affect acquisition of an integrative and conjugative element in Bacillus subtilis. Johnson, CM, Grossman, AD. 2015. Mol. Microbiol. 98, 1222.
    doi: 10.1111/mmi.13282PMID:26767850
  7. Integrative and Conjugative Elements (ICEs): What They Do and How They Work. Johnson, CM, Grossman, AD. 2015. Annu. Rev. Genet. 49, 577-601.
    doi: 10.1146/annurev-genet-112414-055018PMID:26473380
  8. Identification of a Single Strand Origin of Replication in the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis. Wright, LD, Johnson, CM, Grossman, AD. 2015. PLoS Genet. 11, e1005556.
    doi: 10.1371/journal.pgen.1005556PMID:26440206
  9. In Vitro Whole Genome DNA Binding Analysis of the Bacterial Replication Initiator and Transcription Factor DnaA. Smith, JL, Grossman, AD. 2015. PLoS Genet. 11, e1005258.
    doi: 10.1371/journal.pgen.1005258PMID:26020636
  10. Identification of host genes that affect acquisition of an integrative and conjugative element in Bacillus subtilis. Johnson, CM, Grossman, AD. 2014. Mol. Microbiol. 93, 1284-301.
    doi: 10.1111/mmi.12736PMID:25069588
More Publications

Multimedia

Photo credit: Kelly Lorenz