Education
- PhD, 1998, University of California, Los Angeles
- SB, 1992, Physics, Harvard University
Research Summary
Our goal is to understand, at a high level of detail, how the interaction properties of proteins are encoded in their sequences and structures. We investigate protein-protein interactions by integrating data from high throughput assays, structural modeling, and bioinformatics with biochemical and biophysical experiments. Much of our work focuses on α-helical coiled-coil proteins, Bcl-2 apoptosis-regulating proteins, and protein domains that bind to short linear motifs.Recent Publications
- Elaboration of the Homer1 recognition landscape reveals incomplete divergence of paralogous EVH1 domains. Singer, A, Ramos, A, Keating, AE. 2024. Protein Sci 33, e5094.
doi: 10.1002/pro.5094PMID:38989636 - High-throughput discovery of inhibitory protein fragments with AlphaFold. Savinov, A, Swanson, S, Keating, AE, Li, GW. 2024. bioRxiv , .
doi: 10.1101/2023.12.19.572389PMID:38187731 - Marginal specificity in protein interactions constrains evolution of a paralogous family. Ghose, DA, Przydzial, KE, Mahoney, EM, Keating, AE, Laub, MT. 2023. Proc Natl Acad Sci U S A 120, e2221163120.
doi: 10.1073/pnas.2221163120PMID:37098061 - Peptides from human BNIP5 and PXT1 and non-native binders of pro-apoptotic BAK can directly activate or inhibit BAK-mediated membrane permeabilization. Aguilar, F, Yu, S, Grant, RA, Swanson, S, Ghose, D, Su, BG, Sarosiek, KA, Keating, AE. 2023. Structure 31, 265-281.e7.
doi: 10.1016/j.str.2023.01.001PMID:36706751 - Neural network-derived Potts models for structure-based protein design using backbone atomic coordinates and tertiary motifs. Li, AJ, Lu, M, Desta, I, Sundar, V, Grigoryan, G, Keating, AE. 2023. Protein Sci 32, e4554.
doi: 10.1002/pro.4554PMID:36564857 - Tertiary motifs as building blocks for the design of protein-binding peptides. Swanson, S, Sivaraman, V, Grigoryan, G, Keating, AE. 2022. Protein Sci 31, e4322.
doi: 10.1002/pro.4322PMID:35634780 - Native proline-rich motifs exploit sequence context to target actin-remodeling Ena/VASP protein ENAH. Hwang, T, Parker, SS, Hill, SM, Grant, RA, Ilunga, MW, Sivaraman, V, Mouneimne, G, Keating, AE. 2022. Elife 11, .
doi: 10.7554/eLife.70680PMID:35076015 - A distributed residue network permits conformational binding specificity in a conserved family of actin remodelers. Hwang, T, Parker, SS, Hill, SM, Ilunga, MW, Grant, RA, Mouneimne, G, Keating, AE. 2021. Elife 10, .
doi: 10.7554/eLife.70601PMID:34854809 - Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics. Linghu, C, Johnson, SL, Valdes, PA, Shemesh, OA, Park, WM, Park, D, Piatkevich, KD, Wassie, AT, Liu, Y, An, B et al.. 2020. Cell 183, 1682-1698.e24.
doi: 10.1016/j.cell.2020.10.035PMID:33232692 - Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator. Shemesh, OA, Linghu, C, Piatkevich, KD, Goodwin, D, Celiker, OT, Gritton, HJ, Romano, MF, Gao, R, Yu, CJ, Tseng, HA et al.. 2020. Neuron 107, 470-486.e11.
doi: 10.1016/j.neuron.2020.05.029PMID:32592656