Piyush Gupta

Piyush Gupta

Associate Professor of Biology; Member, Whitehead Institute

Piyush Gupta investigates how alterations in cellular differentiation drive cancer malignancy, focusing on the mechanisms that underlie multidrug resistance and invasive progression.

617-324-0086

Phone

WI-661C

Office

Laura Resteghini

Assistant

617-258-7778

Assistant Phone

Education

PhD, 2006, Massachusetts Institute of Technology

Research Summary

We study the ways in which dysregulated cellular differentiation leads to diseases like cancer. Our objective is to understand the cellular and genetic mechanisms that direct self-renewal and differentiation of stem cells in normal tissues and in cancer. To this end, we apply an inter-disciplinary approach involving massively parallel RNAi screening, computational modeling, chemical genetics, gene-expression profiling, next-generation sequencing and in vivo models.

Key Publications

  1. Growth of human breast tissues from patient cells in 3D hydrogel scaffolds. Sokol, ES, Miller, DH, Breggia, A, Spencer, KC, Arendt, LM, Gupta, PB. 2016. Breast Cancer Res. 18, 19.
    doi: 10.1186/s13058-016-0677-5PMID:26926363
  2. De-differentiation confers multidrug resistance via noncanonical PERK-Nrf2 signaling. Del Vecchio, CA, Feng, Y, Sokol, ES, Tillman, EJ, Sanduja, S, Reinhardt, F, Gupta, PB. 2014. PLoS Biol. 12, e1001945.
    doi: 10.1371/journal.pbio.1001945PMID:25203443
  3. Epithelial-to-mesenchymal transition activates PERK-eIF2α and sensitizes cells to endoplasmic reticulum stress. Feng, YX, Sokol, ES, Del Vecchio, CA, Sanduja, S, Claessen, JH, Proia, TA, Jin, DX, Reinhardt, F, Ploegh, HL, Wang, Q et al.. 2014. Cancer Discov 4, 702-15.
    doi: 10.1158/2159-8290.CD-13-0945PMID:24705811
  4. Stochastic state transitions give rise to phenotypic equilibrium in populations of cancer cells. Gupta, PB, Fillmore, CM, Jiang, G, Shapira, SD, Tao, K, Kuperwasser, C, Lander, ES. 2011. Cell 146, 633-44.
    doi: 10.1016/j.cell.2011.07.026PMID:21854987
  5. The melanocyte differentiation program predisposes to metastasis after neoplastic transformation. Gupta, PB, Kuperwasser, C, Brunet, JP, Ramaswamy, S, Kuo, WL, Gray, JW, Naber, SP, Weinberg, RA. 2005. Nat. Genet. 37, 1047-54.
    doi: 10.1038/ng1634PMID:16142232

Recent Publications

  1. BCL11B Drives Human Mammary Stem Cell Self-Renewal In Vitro by Inhibiting Basal Differentiation. Miller, DH, Jin, DX, Sokol, ES, Cabrera, JR, Superville, DA, Gorelov, RA, Kuperwasser, C, Gupta, PB. 2018. Stem Cell Reports 10, 1131-1145.
    doi: 10.1016/j.stemcr.2018.01.036PMID:29503088
  2. Cancer-specific PERK signaling drives invasion and metastasis through CREB3L1. Feng, YX, Jin, DX, Sokol, ES, Reinhardt, F, Miller, DH, Gupta, PB. 2017. Nat Commun 8, 1079.
    doi: 10.1038/s41467-017-01052-yPMID:29057869
  3. 3D Primary Culture Model to Study Human Mammary Development. Miller, DH, Sokol, ES, Gupta, PB. 2017. Methods Mol. Biol. 1612, 139-147.
    doi: 10.1007/978-1-4939-7021-6_10PMID:28634940
  4. SMARCE1 is required for the invasive progression of in situ cancers. Sokol, ES, Feng, YX, Jin, DX, Tizabi, MD, Miller, DH, Cohen, MA, Sanduja, S, Reinhardt, F, Pandey, J, Superville, DA et al.. 2017. Proc. Natl. Acad. Sci. U.S.A. 114, 4153-4158.
    doi: 10.1073/pnas.1703931114PMID:28377514
  5. Cancer cells exhibit clonal diversity in phenotypic plasticity. Mathis, RA, Sokol, ES, Gupta, PB. 2017. Open Biol 7, .
    doi: 10.1098/rsob.160283PMID:28202626
  6. AMPK promotes tolerance to Ras pathway inhibition by activating autophagy. Sanduja, S, Feng, Y, Mathis, RA, Sokol, ES, Reinhardt, F, Halaban, R, Gupta, PB. 2016. Oncogene 35, 5295-5303.
    doi: 10.1038/onc.2016.70PMID:27041569
  7. Growth of human breast tissues from patient cells in 3D hydrogel scaffolds. Sokol, ES, Miller, DH, Breggia, A, Spencer, KC, Arendt, LM, Gupta, PB. 2016. Breast Cancer Res. 18, 19.
    doi: 10.1186/s13058-016-0677-5PMID:26926363
  8. Perturbation-expression analysis identifies RUNX1 as a regulator of human mammary stem cell differentiation. Sokol, ES, Sanduja, S, Jin, DX, Miller, DH, Mathis, RA, Gupta, PB. 2015. PLoS Comput. Biol. 11, e1004161.
    doi: 10.1371/journal.pcbi.1004161PMID:25894653
  9. De-differentiation confers multidrug resistance via noncanonical PERK-Nrf2 signaling. Del Vecchio, CA, Feng, Y, Sokol, ES, Tillman, EJ, Sanduja, S, Reinhardt, F, Gupta, PB. 2014. PLoS Biol. 12, e1001945.
    doi: 10.1371/journal.pbio.1001945PMID:25203443
  10. Epithelial-to-mesenchymal transition activates PERK-eIF2α and sensitizes cells to endoplasmic reticulum stress. Feng, YX, Sokol, ES, Del Vecchio, CA, Sanduja, S, Claessen, JH, Proia, TA, Jin, DX, Reinhardt, F, Ploegh, HL, Wang, Q et al.. 2014. Cancer Discov 4, 702-15.
    doi: 10.1158/2159-8290.CD-13-0945PMID:24705811
More Publications
Photo credit: Gretchen Ertl/Whitehead Institute